Studies on the Respiratory Chain-linked Dihydrodiphosphopyridine Nucleotide Dehydrogenase
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چکیده
منابع مشابه
Studies on the Respiratory Chain-linked Nicotinamide Adenine Dinucleotide Dehydrogenase
Rhein (4,5-dibydroxyanthraquinone-Z-carboxylic acid) inhibits the mitochondrial oxidation of reduced nicotamide adenine dinucleotide (NADH) but not of succinate. The inhibition is competitive with respect to substrate and involves a block between NADH and the flavin of NADH dehydrogenase. The Ki is about 2 ~.LM at 30”. Evidence for this localization has come from kinetic analysis of the effect ...
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The finding that ferredoxin and photosynthetic pyridine nucleotide reductase contain approximately equimolar amounts of “labile” sulfide and nonheme iron, and that both of these components are liberated in inorganic form with attendant inactivation upon acid treatment of these proteins (3-5), has stimulated considerable interest in the chemical nature and function of labile sulfide in oxidizing...
متن کاملStudies on the Respiratory Chain-linked Reduced Nicotinamide Adenine Dinucleotide Dehydrogenase XI. TRANSFORMATION OF THE DEHYDROGENASE TO REDUCED NICOTINAMIDE ADENINE
Purified preparations of the reduced nicotinamide adenine dinucleotide dehydrogenase of the respiratory chain, extracted with the aid of phospholipase at moderate temperature, do not catalyze the reduction of long chain coenzyme Q derivatives (Q6 and QIO) at significant rates and are not inhibited by amytal or rotenone. On exposure of the enzyme to conditions which have been used for the extrac...
متن کاملStudies on the Respiratory Chain-linked Reduced Nicotinamide Adenine Dinucleotide Dehydrogenase XIII. BINDING SITES OF ROTENONE, PIERICIDIN A, AND AMYTAL IN THE RESPIRATORY CHAIN*
The binding sites of rotenone, piericidin A, and Amytal in the reduced nicotinamide adenine dinucleotide oxidase chain of heart have been studied with the aid of rotenone6aJ4C. Binding of rotenone continues in a linear manner beyond the point of maximal inhibition of respiration, indicating that rotenone is tightly bound not only at the specific site in the NADH dehydrogenase segment of the res...
متن کاملStudies on the respiratory chain-linked reduced nicotinamide adenine dinucleotide dehydrogenase. 13. Binding sites of rotenone, piericidin A, and amytal in the respiratory chain.
The binding sites of rotenone, piericidin A, and Amytal in the reduced nicotinamide adenine dinucleotide oxidase chain of heart have been studied with the aid of rotenone6aJ4C. Binding of rotenone continues in a linear manner beyond the point of maximal inhibition of respiration, indicating that rotenone is tightly bound not only at the specific site in the NADH dehydrogenase segment of the res...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1962
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)93963-3